Turtle Eggs And Nesting

After you are certain that your turtles have mated, you should create a nesting area for the female.  It should be wide, with 6 to 12 inches of light, moist soil.  Here is a great video on how to prepare a nesting area for her:
 

How Long Until My Female Turtle Lays Eggs?  How Will I know When She Is Ready?
It can be days or even weeks after mating, until the female lays her eggs.   You should watch for signs that the female is ready to lay eggs.   She will usually start basking under the UV lights a lot more.   Acting very restless.  Frantically trying to get out of the tank (she wants to go look for a nesting area).  Making strange grunting or sputtering noises.   Digging with her front and back feet.  
You can also check your female to see if she has eggs.   To do this, gently feel in the area between her top and bottom shell, above her back legs.   If you feel round bumps in there, she probably has eggs.  One good way to know the difference is to try it on your male first, to see what “no eggs” feels like. Then try it on the female, and if it feels like she has round lumps in there while he does not—then the female probably does have eggs in there.  
How often Do female Turtles Lay Eggs, And How Many?
The female may accidentally lay an egg in the water.  If this happens, put her in a nesting area.   Eggs laid in the water will usually die, but you can try to incubate it as soon as possible.  Try not to turn or rotate the egg when you pick it up (this can kill the embryo), keep it as level as possible.  
Incubation takes 55 to 60 days for most average-sized aquatic breeds.   
A female turtle can lay 2 to 30 eggs, depending on body size and other factors.   A “clutch” is the group of eggs that she lays.   She can lay up to 5 clutches in one year, 12 to 36 days apart.  
Keep in mind though, females will lay eggs even if they have never met a male.  And even if they have mated, not all eggs will be fertile.  
Fertilizing Eggs
Females can store male sperm for weeks, months, even 2 to 3 years after mating, and use it to fertilize their eggs.   The actual fertilizing takes place when the eggs are being laid.  If the female has mated, and the eggs are covered in a slimy substance, there is a good chance they were fertilized.  
Incubating Eggs
Before you do the below steps, you will want to have your incubator set up and ready.   Here is a video on how you can make your own, and it also has a shot of an incubator you can purchase from a store:   

You will also want to have some plastic containers ready to put the eggs in.  The containers should be filled with a very light, moistened potting soil mixed with vermiculite.  Keep these close by.
You will probably need to dig up the eggs after they are laid, so you can transfer them to your containers and put them in the incubator.   But you must do this very gently, gradually moving the nesting box dirt away with your hands until you find the eggs.   Clear the dirt away from each egg carefully, without moving them or breaking them.   Mark the tops of the eggs with an X using a felt pen and being sure not to rotate them at all, during moving.   
In your egg containers, make small dents in the soil to place each egg in.  The bottom half of the egg should be covered in the soil.  The top half of the egg should be showing.  Here is a video demonstrating this:

Now you should place your egg container inside your egg incubator.  
Incubator Temperatures And Humidity:
What temperature you keep the eggs at really depends on what gender you want.  Some people like to keep one incubator for female eggs, and one for male eggs.   
79.88 degrees F (26.6 c)—Male
87.8 degrees F (31 c) –Female
Somewhere in between can produce equal amounts of both.  But many people like to separate them, so they will know which gender is which from birth.  Otherwise, you won’t be able to determine their gender until their shells are 4 inches long…
In the wild, clutches of eggs laid in cooler weather will usually turn out to be all male.  Clutches laid in warmer weather will be all female.   And clutches laid in neutral weather can be 50/50. 

In your home made incubator, you will probably have the eggs resting on a platform, above a few inches of water, with an aquarium water heater under the water.  Keeping the water heater set to 78 to 83 degrees F, will also maintain a humidity level of 75 to 85 %( Midland eggs are leathery and will dent or collapse and die if relative humidity is not maintained at 80% or better.).  
The eggs should hatch between 50 to 60 days.   
How To Tell If Turtle Eggs Are Fertile
There is a stronger chance of fertile eggs if they are covered in a slimy substance when they are laid.   But there are also other ways to tell after they have been laid— you will see a white ring (whiter than most of the shell) forming around the middle of the egg.  This is called “banding” because its like a band around the middle.   Eventually, that whiter color will spread all over the whole egg.  This is a good sign that the eggs are fertile.  
Some people also “candle” their eggs ( gently picking one up without rotating or cracking it, and holding it over a candle or flashlight to look for veins or masses growing inside).   But this method is not really advisable, because there is too much risk of killing the baby, or cracking the egg, while moving it…   
For information on what to do with baby turtles after they have hatched, please see my post on “Caring For Baby Turtles / Hatchlings”.

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